Ten Commandments: Lessons from the Enzymology of DNA Replication

Ten commandments of enzymology, amended.

Reflections on advances and retreats in biochemistry have, over the years, generated several maxims. The late Efraim Racker’s ‘don’t waste clean thinking on dirty enzymes’ has been one of the most durable. On the occasion of a lecture in Jerusalem a decade ago, I framed a list of the ten ‘best’ maxims as ‘commandments’, which were later published as a ‘Guest Commentary’ in the Journal of Bacter...

The Enzymology of DNA Replication

4. SEALING OF DNA FRAGMENTS 219 4.1. E. coli DNA ligase 4.2. Bacteriophage T 4 gene 30 protein 4.3. Mammalian DNA ligases I and II C O N F O R M A T I O N A L CHANGES OF DNA DURING REPLICATION 220 5.1. E. coli HDP 5.2. Bacteriophage-coded HDPs — T 4 gene 32 protein —T7 HDP —fd gene V protein 5.3. Eukaryotic HDPs 5.4. Bacterial DNA topoisomerases —Swivelases —DNA gyrase 5.5. Bacteriophage-coded ...

The Ten Commandments.

DNA replication: enzymology and mechanisms.

Research into the enzymology of DNA replication has seen a multitude of highly significant advances during the past year, in both prokaryotic and eukaryotic systems. The scope of this article is limited to chromosomal replicases and origins of initiation. The multiprotein chromosomal replicases of prokaryotes and eukaryotes appear to be strikingly similar in structure and function, although fut...

Ten Commandments of Hypospadias Surgery

Submit Manuscript | http://medcraveonline.com penile in 77(30%) and proximal Hypospadias in 46(18%) patients. Tubularized incised plate urethroplasty (TIP) by Dr. Warren Snodgrass and Byar’s two stage urethroplasty were the only two basic methods applied. Only 13 patients (5%) of total required two stage repairs. Subsequent 10 years, I operated 512 cases of hypospadias with the Ten Commandments...